T8660
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 | Monoclonal Anti-b-Tubulin, Isotype III, Clone SDL-3D10 (T 8660) Immunoblot Whole cell extract of rat brain cytosol was separated on SDS-PAGE and blotted with Monoclonal Anti-b-Tubulin Isotype III (Product No. T 8660). The antibody was developed with Goat anti-Mouse IgG, Peroxidase conjugate (Product No. A 9917) and AEC substrate. Lane A: Antibody dilution 1:400 Lane B: Negative control (only secondary antibody) |
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T8660
Sigma
Monoclonal Anti-β-Tubulin III antibody produced in mouse
clone SDL.3D10, ascites fluid
| MDL number: | MFCD01323870 |
Description
| Immunogen | synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype III coupled to BSA |
| Specificity | The antibody specifically recognizes an epitope located on isotype III of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. The antibody can stimulate microtubule assembly when reconstituted with tubulin and either tau or MAP2. |
Properties
| antibody form | ascites fluid |
| clone | SDL.3D10 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | rat, human, bovine |
| application(s) | immunoblotting: 1:400 using a rat brain extract |
| indirect ELISA: suitable | |
| isotype | IgG2b |
| Gene Information | human ... TUBB3(10381) |
| rat ... Tubb3(246118) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | Banerjee, A., et al., A monoclonal antibody against the type II isotype of β-tubulin. Preparation of isotypically altered tubulin. J. Biol. Chem. 263, 3029, (1988) Abstract |
| Banerjee, A., et al., Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of β-tubulin. J. Biol. Chem. 265, 1794, (1990) Abstract | |
| Diego Gomez-Nicola, Beatriz Valle-Argos, D. Wolfgang Pita-Thomas, Manuel Nieto-Sampedro, Interleukin 15 Expression in the CNS: Blockade of Its Activity Prevents Glial Activation After an Inflammatory Injury Glia 56, 494-505, (2008) Abstract | |
| Botkjaer, A., et al., A role for docosahexaenoic acid–derived neuroprotectin D1 in neural cell survival and Alzheimer disease J. Clin. Invest. 115, 2774-2783, (2005) Abstract | |
| Banerjee, A., et al., Direct involvement of the isotype-specific C-terminus of β-tubulin in ciliary beating J. Cell Sci. 118, 4333-4341, (2005) Abstract | |
| Zeng, a., et al., Gene Expression Profile of Neuronal Progenitor Cells Derived from hESCs: Activation of Chromosome 11p15.5 and Comparison to Human Dopaminergic Neurons PLoS ONE 3, e1422, (2008) Abstract |
